Inclusions are internal features within a gemstone, often small enough to require a microscope for viewing. This term usually refers to foreign materials in gemstones like guest crystals, fluids and gasses. Gemologists also use the term inclusion describe phenomena, that don't comprise foreign substances, such as color zoning, twinning and phantoms.
Inclusions can end up in gemstones in several ways:
The internal world of gemstones is of great value to gemologists who can derive critical information from observing inclusions. The presence or absence of some inclusions can provide diagnostic clues for those are trained to interpret them. Inclusions are essential in separating natural gemstones from synthetic (lab grown) crystals.
Edward Gübelin and John Koivula are two preeminent gemologists who have published a series of indispensable books on inclusion studies that every gem fanatic should read: Photoatlas of Inclusions in Gemstones, Volumes 1, 2 & 3.